One of the most commonly used clinical tests performed today is the routine evaluation of peripheral blood smears. In this paper, we investigate the design, development, and implementation of a robust color gradient vector flow (GVF) active contour model for performing segmentation, using a database of 1791 imaged cells. The algorithms developed for this research operate in Luv color space, and introduce a color gradient and 2 robust estimation into the traditional GVF snake. The accuracy of the new model was compared with the segmentation results using a mean-shift approach, the traditional color GVF snake, and several other commonly used segmentation strategies. The unsupervised robust color snake with 2 robust estimation was shown to provide results which were superior to the other unsupervised approaches, and was comparable with supervised segmentation, as judged by a panel of human experts.